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题目:: Hela Mitotic Selection - gene expression time series in early G1 phase of the human cell cycle
ID:
状态:: 发布时间June 25, 2010 , 更新时间 May 1, 2014 , 提交时间 Aug. 17, 2008,
物种:: Homo sapiens
摘要:: HeLa mitotic cells were collected using a mitotic shakeoff apparatus to study gene expression in early G1 phase of the human cell cycle. This set includes microarray data from two shake-off experiments : shake 1 (0h-14h) which was conducted over 14 hours and RNA samples collected every 2 hrs, and shake 2 (0min-120min) which was conducted over 2 hours and samples collected every 15 minutes. Total RNA was prepared using ULTRASPEC RNA isolation system, and Reference RNA was extracted from asynchronously growing HeLa cells using TRIzol. For cDNA synthesis and microarray hybridization, refer to Whitfield ML. et al., Mol Biol Cell, 2002. 13(6): p. 1977-2000. Groups of assays that are related as part of a time series. Age: g1 progression after mitotic shake-off Replicate: two biological replicates (shake1 and shake2) Keywords: time_series_design Computed
实验种类:: transcription profiling by array
样本量:: 34
实验设计:
: 无设计数据
数据号:: E-GEOD-12473, GSE12473
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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