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题目:: Global transcriptional profiling of Saccharomyces cerevisiae treated with allicin
ID:
状态:: 发布时间June 23, 2008 , 更新时间 May 1, 2014 , 提交时间 June 21, 2008,
物种:: Saccharomyces cerevisiae
摘要:: We employed CapitalBio Corporation to investigate the global transcriptional profiling of Saccharomyces cerevisiae treated with allicin. S. cerevisiae strain L1190 was incubated in a rotary shaker. Subsequently, allicin was added to the culture and DMSO to the control. Then, the yeast cells were further incubated for a certain time and total RNA was extracted by a hot acidic phenol method. The experimental sample and control sample were incorporated and dissolved in hybridization solution after labelled. Arrays hybridization was preformed in a CapitalBio BioMixerTM II Hybridization Station overnight and washed with two consecutive solutions. Arrays were scanned with a confocal LuxScanTM scanner and the images obtained were then analyzed using LuxScanTM 3.0 software. A space- and intensity-dependent normalization based on a LOWESS program was employed.
实验种类:: transcription profiling by array
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-11856, GSE11856
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