| GeneLibs

题目:: Transcription profiling of mouse wild type, IFNg-/- and IRF1-/- animals infected with from M. avium
ID:
状态:: 发布时间Oct. 25, 2008 , 更新时间 June 10, 2011 , 提交时间 June 17, 2008,
物种:: Mus musculus
摘要:: Mycobacterium avium infection in mice induces granuloma necrosis in the lung which is dependent on IFNg. IRF1 is a transcription factor activated by IFNg signaling. The effect of IFNg and IRF1 on immunopathology and transcriptional changes in the lung were analysed using gene-deficient mice. The data from the Ifng-/- experiment have been described (Aly et al. 2007. J Pathol 212:295). Experiment Overall Design: In two independent blocks of experiments, Ifng-/- and Irf1-/- mice on a C57Bl/6 background and their respective controls were infected with Mycobacterium avium via aerosol. After 14 weeks, mice were sacrificed and the lung RNA prepared using TriFast FL (Peqlab). Total RNA (1µg) was labeled and hybridized to Affymetrix mouse MOE430A 2.0 GeneChips according to the manufacturer’s recommendations. For each condition three biological replicates (except Ifng-/-, no infection) were used. Total of 23 Samples.
实验种类:: transcription profiling by array
样本量:: 23
实验设计:
: 无设计数据
数据号:: E-GEOD-11809, GSE11809
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息