| GeneLibs

题目:: CEA_SGF:E00004#TetOFF_Stat3F
ID:
状态:: 发布时间July 1, 2004 , 更新时间 March 27, 2012 , 提交时间 March 26, 2004,
物种:: Mus musculus
摘要:: The goal of this study was to identify the target genes of Stat3 involved in murine ES cell self-renewal. To achieve this goal, we used the Gs2 ES cell line expressing an inducible dominant negative mutant of Stat3 (Stat3F) in wich Y705 is mutated to phenilalanine. The Gs2 ES cells are routinely maintained in the presence of LIF 1000 U/ml and tetracycline 1µg/ml (LIF/ON). In this condition, Stat3F is not expressed and the cells are maintained in an undifferentiated phenotype. Upon tetracycline removal but inthe presence of LIF (Tet OFF condition) Stat3F is expressed and the cells differentiate despite the presence of LIF. We thus performed microarray analysis of the transcriptome of ES cells after 16h, 24h and 48h after Tetracycline removal (OFF16; OFF24 and OFF48) respectively)and compared them to Gs2 ES cells maintained in the continuous presence of LIF and tetracyclin (LIF/ON).
实验种类:: unknown experiment type
样本量:: 46
实验设计:
: 无设计数据
数据号:: E-GEOD-1151, GSE1151
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息