| GeneLibs

题目:: shRNAi profiling of huuman CHD8 depleted cells
ID:
状态:: 发布时间May 14, 2009 , 更新时间 May 2, 2014 , 提交时间 May 12, 2008,
物种:: Homo sapiens
摘要:: CHD8 is a putative chromatin remodeling ATPase of the SNF2 family. We found that depletion of CHD8 impairs cell proliferation. In order to identify CHD8 target genes, we performed a transcriptomic analysis of CHD8-depleted cells. CHD8 was knockdown by doxycyclin-dependent expression of a shRNA that target the CHD8 mRNA. Experiment Overall Design: C33KD2 is a stable cell line where expression of a shRNA, that targets the CHD8 mRNA, is dependent on doxycycline. Addition of doxyclycin provokes a significant reduction in the level of CHD8 protein. Total RNA samples coming from C33KD2 cells cultured for 48 hours either with or without 2 µg/ml doxycycline were used to generate complementary RNA labelled with Cy5 with the Agilent Low RNA Input Fluorescent Linear Amplification Kit (Agilent Technologies). The reference sample was Stratagene human universal reference and was amplified at the same time as the sample RNA using the same method. Reference samples were labeled using Cy3.
实验种类:: RNAi profiling by array
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-GEOD-11422, GSE11422
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息