RNAi profiling of mouse hippocampal neurons infected with lentivirus expressing Npas4-RNAi or control-RNAiNpas4-regulated genes
ID:
状态:
发布时间Oct. 26, 2008
, 更新时间 May 2, 2014
, 提交时间 April 24, 2008,
物种:
Mus musculus
摘要:
we performed a DNA microarray experiment to identify activity-regulated genes that are misregulated in the absence of Npas4. Experiment Overall Design: We infected mouse hippocampal neurons with lentivirus expressing Npas4-RNAi or control-RNAi @ 3 DIV and depolarized the neurons @ 8 DIV with 50 mM of KCl for 0, 1, 3 or 6 hours. Neurons were lysed, mRNA isolated and hybridized to Affymetrix arrays. Data were collected from 3 independent experiments.