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题目:: Transcription profiling of mouse masticatory and limb skeletal muscle
ID:
状态:: 发布时间June 20, 2008 , 更新时间 June 10, 2011 , 提交时间 April 9, 2008,
物种:: Mus musculus
摘要:: Distinctions between craniofacial and axial muscles exist from the onset of development and throughout adulthood. The masticatory muscles are a specialized group of craniofacial muscles that retain embryonic fiber properties throughout adulthood, suggesting that the developmental origin of these muscles may govern a pattern of expression that differs from limb muscles. To determine the extent of these differences, expression profiling of total RNA isolated from the masseter and tibialis anterior (TA) muscles of adult female mice was performed, which identified transcriptional changes in unanticipated functional classes of genes in addition to those associated with fiber type. In particular, the masseters displayed a reduction of transcripts associated with load-sensing and anabolic processes, and heightened expression of genes associated with stress. Consistent with these observations were a significantly smaller fiber cross-sectional area in masseters, significantly elevated load-sensing signaling (phosphorylated Focal Adhesion Kinase (FAK)), and increased apoptotic index in masseters compared to TA muscles. Based on these results, we hypothesize that masticatory muscles may sense and respond to load differently than limb muscles, where the drive for anabolic processes is reduced, and cell stress mediated processes are enhanced. These results establish a novel classification for the masseter muscle in the spectrum of skeletal muscle allotypes, and may provide insight into the molecular basis for specific muscle-related pathologies associated with masticatory muscles. Experiment Overall Design: Tissues were isolated from normal adult female mice (C57Bl/6), age 6 months. Paired comparisons between masseter and tibialis anterior muscles were performed on all present genes using "Significance Analysis of Microarrays" (SAM) to identify differentially expressed genes between masticatory and axial muscles.
实验种类:: transcription profiling by array
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-11114, GSE11114
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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