Transcription profiling of mouse colon from wild-type and Slc9a3 (NHE3)-deficient animals
ID:
状态:
发布时间April 6, 2008
, 更新时间 June 10, 2011
, 提交时间 March 5, 2008,
物种:
Mus musculus
摘要:
Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global programme of colonic gene expression in the absence of functional NHE3. Experiment Overall Design: Whole colon was dissected from 6-8 week old NHE3-deficient mice and their wild-type littermates and total RNA isolated for microarray analysis using Affymetrix murine MOE430 2.0 arrays