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题目:: Global Transcriptional Response of Nitrosomonas europaea to Cyanide
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状态:: 发布时间May 26, 2010 , 更新时间 June 10, 2011 , 提交时间 Feb. 28, 2008,
物种:: Nitrosomonas europaea
摘要:: Pure cultures of ammonia oxidizing bacterium, Nitrosomonas europaea, are exposed to cyanide in pseudo-steady state batch reactor in presence of ammonia. Nitrosomonas europaea are generally regarded as the most sensitive organism to various inhibitors commonly encountered in the wastewater treatment plants (WWTP). To find stress genes of Nitrosomonas europaea to cyanide known as inhibitor of respiratory process, whole-genome transcript response to cyanide was determined in this research using microarray and qRT-PCR. When 1 uM NaCN inhibits nitrification about 50 %, transcript levels of 35 genes were increased while transcript levels of 29 genes were decreased, showing more than 2-fold in total 2460 genes. moeZ (NE2353), homologue with rhodanases related to detoxification of CN-, showed 7-fold up regulation and gene cluster including moeZ also showed significant up regulation. Keywords: cyanide, stress response, moeZ The 1 uM NaCN caused more than 50 % inhibition in physiological response for 1 hour incubation. Transcriptional levels of the cells inhibited by cyanide were compared with the cells under control condition.
实验种类:: transcription profiling by array
样本量:: 6
实验设计:
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数据号:: E-GEOD-10664, GSE10664
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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