实验库 数据相关信息
- 题目:
- Gene expression profiling of caffeic acid phenethyl ester-treated human umbilical vein endothelial cells-1
- ID:
- 状态:
- 发布时间May 26, 2010 , 更新时间 May 2, 2014 , 提交时间 Feb. 6, 2008,
- 物种:
- Homo sapiens
- 摘要:
- Caffeic acid phenethyl ester (CAPE), derived from various plant sources, has been shown to ameliorate ischemia/reperfusion (I/R) injury in vivo, and this has been attributed to its ability to reduce the oxidative stress. Here we investigated the cytoprotection of CAPE against menadione (MD)-induced oxidative stress in human umbilical vein endothelial cells (HUVEC) to evaluate potential gene expression involvement. CAPE exhibited dose-dependent cytoprotection of HUVEC that required preincubation. A gene screen with microarrays was performed to identify the potential cytoprotective gene(s) induced by CAPE. Heme oxygenase-1 (HO-1) was highly upregulated by CAPE and this was confirmed with reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting. Keywords: Gene expression in HUVEC, CAPE cytoprotective dose response Confluent HUVEC were incubated with cytoprotective dose of CAPE at 5 µg/ml or 0.1% DMSO as vehicle control for 6 hrs. Both treatments were done in triplicates. Total RNA was isolated at the end of the treatment and applied to microarray experiments in order to identify transcriptional response of HUVEC to CAPE. Microarray experiments were based on a two-color reference design using human universal reference RNA to compare results bwtween CAPE treatment and vehicle control groups.
- 实验种类:
- unknown experiment type
- 样本量:
- 12
- 实验设计:
- 无设计数据
- 数据号:
- E-GEOD-10413, GSE10413
- 数据状态:
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DataTable pData
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