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题目:: In vitro dormancy achieved by multiple stresses in Mycobacterium tuberculosis
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状态:: 发布时间May 1, 2008 , 更新时间 May 2, 2014 , 提交时间 Feb. 5, 2008,
物种:: Mycobacterium tuberculosis H37Rv
摘要:: The human pathogen, Mycobacterium tuberculosis, develops a dormant infection, in which organisms survive within the body. We established a unique in vitro dormancy model based on the characterization of drug-resistance to INH and rifampin. M. tuberculosis cells were maintained in controlled and defined multiple stress conditions with low oxygen (5% dissolved oxygen tension), acid (pH 5.) along with glycerol-deprived medium conditions. To monitor gene expression changes in M. tuberculosis in response to the multiple stresses, we performed microarray analysis at the time point of 1, 2, 3, 6, and 12days after treatment. M. tuberculosis adapting to multiple stresses displayed characteristics associated with persistence in vivo, including entry into a non-replicative state and the repression of genes involved in energy regeneration. Under in vitro multiple-stresses, M. tuberculosis significantly modulated gene expression mainly in response to the starvation stresses. Cells exposed to these multiple stress conditions shows significant drug-resistance. Comparison with other in vivo expression profiles demonstrates induction of several common genes for in vitro dormancy conditions. Keywords: Stress response in time course. Samples under multiple stress condition were taken at day 1, 2, 3, 6, and 12 for microarray hybridization. More than two technical replicates per biological samples with Cy3/5 dye-swaps.
实验种类:: transcription profiling by array
样本量:: 150
实验设计:
: 无设计数据
数据号:: E-GEOD-10391, GSE10391
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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