Single cell gene expression patterns across different cell cycle stages in mouse embryonic cells

·发布 2014年4月28日 ·更新 2016年3月1日

388

样本数

1540

实验数

实验描述

This study is aimed to decipher the gene expression patterns that exist across the different cell cycle stages at level of individual cells and their inherent heterogeneity. Conventional studies in yeast and human cells have shown coordination between gene expression and cell-cycle. However such a study for mouse ES cells (mES) and their differentiation has not been performed at a single cell level. In this study, we obtain pure mES cells from different cell cycle stages using FACS and apply single cell RNA-sequencing using the Fluidigm C1 system. This work will decipher and unravel the transcriptional patterns that coordinated with cell cycle progression at a single cell level.

样本属性

cell type

ESC

developmental stage

embryonic stem cell line

genotype

AB2.2. Rex1-GFP

growth condition

2i/LIF

organism

Mus musculus

sample description

AB2.2 Rex1-GFP cells. Asynchronous cells stained with 5uM Hoechst staining and sN/Ap frozen on 9/2/2014. RN/A extraction done by Sequencing facility, AB2.2 Rex1-GFP cells. Cells sorted using 5uM Hoechst staining for G1 phase and sN/Ap frozen on 9/2/2014. RN/A extraction done by Sequencing facility, AB2.2 Rex1-GFP cells. Cells sorted using 5uM Hoechst staining for G2M phase and sN/Ap frozen on 9/2/2014. RN/A extraction done by Sequencing facility, AB2.2 Rex1-GFP cells. Cells sorted using 5uM Hoechst staining for S phase and sN/Ap frozen on 9/2/2014. RN/A extraction done by Sequencing facility, mRNAseq Nextera library made from AB2.2 Rex1-GFP. Cells sorted using 5uM Hoechst staining for G1 phase and lysed on 5/12/2013, mRNAseq Nextera library made from AB2.2 Rex1-GFP. Cells sorted using 5uM Hoechst staining for G1 phase and lysed on 6/12/2013, mRNAseq Nextera library made from AB2.2 Rex1-GFP. Cells sorted using 5uM Hoechst staining for G2M phase and lysed on 5/12/2013, mRNAseq Nextera library made from AB2.2 Rex1-GFP. Cells sorted using 5uM Hoechst staining for S phase and lysed on 6/12/2013

sex

male

strain

AB2.2. Rex1-GFP

实验信息

登记号

E-ERAD-240

GEO 编号

ERP004534

实验类型

RNA-seq of coding RNA

物种

Mus musculus

发布日期

2014年4月28日

更新日期

2016年3月1日

提交者

The Wellcome Trust Sanger Institute

外部链接

ArrayExpress 原始页 NCBI GEO：ERP004534

分析服务

Single cell gene expression patterns across different cell cycle stages in mouse embryonic cells

联系地址

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