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PMID: 9696778 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of the puc operon promoter from Rhodobacter capsulatus.

Journal of bacteriology ·Vol. 180 ·No. 16 ·1998-08-00 ·Pages 4270-7

Nickens DG, Bauer CE

Abstract

Expression of the Rhodobacter capsulatus puc operon, which codes for structural polypeptides of the light-harvesting-II peripheral antenna complex, is highly regulated in response to alterations in oxygen tension and light intensity. To obtain an understanding of the puc promoter region we report the high-resolution 5' mapping of the puc mRNA transcriptional start site and DNA sequence analysis of the puc upstream regulatory sequence (pucURS). A sigma70-type promoter sequence was identified (pucP1) which has a high degree of sequence similarity with carotenoid and bacteriochlorophyll biosynthesis promoters. Inspection of the DNA sequence also indicated the presence of two CrtJ and four integration host factor (IHF) binding sites. Transcriptional fusions of the pucURS fused to lacZ also confirmed that puc promoter activity is regulated by the transcriptional regulators IHF, CrtJ, and RegA. Gel retardation analysis using cell extracts indicates that mutations in IHF and RegA disrupt protein binding to DNA fragments containing the pucURS.

MeSH Terms
Base Sequence DNA, Bacterial Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Reporter Light-Harvesting Protein Complexes Molecular Sequence Data Operon Photosynthetic Reaction Center Complex Proteins/genetics Promoter Regions, Genetic Rhodobacter capsulatus/genetics Sequence Alignment
Chemicals
DNA, Bacterial Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nickens D G
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
Bauer C E
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.16.4270-4277.1998
Published
1998-08-00
Pages
4270-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107426
Subset
IM
Grants
NIGMS NIH HHS · GM40941 · United States
NIGMS NIH HHS · R01 GM040941 · United States
NIGMS NIH HHS · R37 GM040941 · United States
NIGMS NIH HHS · GM53940 · United States
NIGMS NIH HHS · GM00618 · United States
NIGMS NIH HHS · R01 GM053940 · United States
Databases
GENBANK
AF031407
External Links
PubMed source DOI: 10.1128/JB.180.16.4270-4277.1998 PMC Full Text View journal details
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