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PMID: 7989547 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Typing of group A streptococci by random amplified polymorphic DNA analysis.

Journal of clinical microbiology ·Vol. 32 ·No. 8 ·1994-08-00 ·Pages 1945-8

Seppälä H, He Q, Osterblad M, Huovinen P

Abstract

Random amplified polymorphic DNA (RAPD) analysis was evaluated in comparison with restriction endonuclease analysis (REA) of genomic DNA and serotyping in the typing of 160 epidemiologically unrelated group A streptococci (GAS). Amplification of genomic DNA of GAS was performed with a single primer with an arbitrarily selected nucleotide sequence of 12 nucleotides. In total, 31 RAPD patterns and 15 REA patterns were observed among the isolates studied. The results of RAPD analysis were in accordance with the results of REA for 86% of the isolates, as both methods identified 15 different strains among 138 isolates. However, RAPD analysis differentiated 16 additional strains among 22 isolates. RAPD analysis was somewhat better than REA for differentiation of isolates of the same and different serotypes. However, not all of the serotypes were differentiated by RAPD analysis either. In conclusion, RAPD analysis provides a practical alternative for genomic typing of GAS. It can be recommended for the typing of GAS, especially if used in parallel with serotyping.

MeSH Terms
Bacterial Typing Techniques DNA Primers DNA, Bacterial/genetics Molecular Sequence Data Polymerase Chain Reaction/methods Polymorphism, Genetic Polymorphism, Restriction Fragment Length Serotyping Streptococcus pyogenes/classification,genetics
Chemicals
DNA Primers DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Seppälä H
Antimicrobial Research Laboratory, National Public Health Institute, Turku, Finland.
He Q
Osterblad M
Huovinen P
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22 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.32.8.1945-1948.1994
Published
1994-08-00
Pages
1945-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC263908
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.32.8.1945-1948.1994 PMC Full Text View journal details
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