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PMID: 7638185 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 16 ·1995-08-01 ·Pages 7302-6

Fukuda T, Kitamura D, Taniuchi I, Maekawa Y, Benhamou LE, Sarthou P, Watanabe T

Abstract

The HS1 protein is one of the major substrates of non-receptor-type protein-tyrosine kinases and is phosphorylated immediately after crosslinking of the surface IgM on B cells. The mouse B-lymphoma cell line WEHI-231 is known to undergo apoptosis upon crosslinking of surface IgM by anti-IgM antibodies. Variants of WEHI-231 that were resistant to anti-IgM-induced apoptosis expressed dramatically reduced levels of HS1 protein. Expression of the human HS1 protein from an expression vector introduced into one of the variant cell lines restored the sensitivity of the cells to apoptosis induced by surface IgM crosslinking. These results suggest that HS1 protein plays a crucial role in the B-cell antigen receptor-mediated signal transduction pathway that leads to apoptosis.

MeSH Terms
Adaptor Proteins, Signal Transducing Animals Apoptosis/genetics,immunology Blood Proteins/genetics,immunology,metabolism Gene Expression Genetic Variation Humans Immunoglobulin M/metabolism Lymphoma, B-Cell/genetics,immunology,pathology Mice Protein-Tyrosine Kinases/metabolism Receptors, Antigen, B-Cell/metabolism Signal Transduction Substrate Specificity Tumor Cells, Cultured
Chemicals
Adaptor Proteins, Signal Transducing Blood Proteins HCLS1 protein, human Immunoglobulin M Receptors, Antigen, B-Cell Protein-Tyrosine Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fukuda T
Department of Molecular Immunology, Kyushu University, Fukuoka, Japan.
Kitamura D
Taniuchi I
Maekawa Y
Benhamou L E
Sarthou P
Watanabe T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.16.7302
Published
1995-08-01
Pages
7302-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41327
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.16.7302 PMC Full Text View journal details
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