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PMID: 2109575 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Outer membrane protein D2 catalyzes facilitated diffusion of carbapenems and penems through the outer membrane of Pseudomonas aeruginosa.

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 1 ·1990-01-00 ·Pages 52-7

Trias J, Nikaido H

Abstract

The outer membrane of imipenem-resistant mutants of Pseudomonas aeruginosa with decreased permeability to imipenem was shown by Western (immuno-) blotting to contain protein D1 and to lack protein D2. Protein D2 was purified and was shown to allow the permeation of imipenem at a rate higher than expected from its molecular weight. Spontaneous imipenem-resistant mutants of P. aeruginosa PAO1 appeared at a frequency of 10(-8) in the laboratory and did not synthesize protein D2. Experiments performed with intact cells carrying plasmid pHN4 containing the gene for L-1 beta-lactamase from Pseudomonas maltophilia showed that this channel could also be used by SM-7338, Sch 33755, and Sch 33440 but apparently not by Sch 34343 or Sch 29482.

MeSH Terms
Anti-Bacterial Agents/metabolism Bacterial Outer Membrane Proteins/metabolism Cell Membrane Permeability Diffusion Imipenem/pharmacology Kinetics Lactams Meropenem Molecular Weight Plasmids Pseudomonas aeruginosa/genetics,metabolism,ultrastructure Thienamycins/metabolism
Chemicals
Anti-Bacterial Agents Bacterial Outer Membrane Proteins Lactams Thienamycins Imipenem Sch 33755 Sch 29482 Sch 33440 Sch 34343 Meropenem
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Trias J
Department of Microbiology and Immunology, University of California, Berkeley 94720.
Nikaido H
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.1.52
Published
1990-01-00
Pages
52-7
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171519
Subset
IM
Grants
NIAID NIH HHS · AI-09144 · United States
External Links
PubMed source DOI: 10.1128/AAC.34.1.52 PMC Full Text View journal details
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