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题目:: Multiple alternative splicing markers for ovarian cancer.
作者:: Klinck(Roscoe),Bramard(Anne),Inkel(Lyna),Dufresne-Martin(Geneviève),Gervais-Bird(Julien),Madden(Richard),Paquet(Eric R),Koh(ChuShin),Venables(Julian P),Prinos(Panagiotis),Jilaveanu-Pelmus(Manuela),Wellinger(Raymund),Rancourt(Claudine),Chabot(Benoit),Abou Elela(Sherif)
状态:: 发布时间2008-02-04 , 更新时间 2008-02-04
期刊:: Cancer Res
摘要:: Intense efforts are currently being directed toward profiling gene expression in the hope of developing better cancer markers and identifying potential drug targets. Here, we present a sensitive new approach for the identification of cancer signatures based on direct high-throughput reverse transcription-PCR validation of alternative splicing events. This layered and integrated system for splicing annotation (LISA) fills a gap between high-throughput microarray studies and high-sensitivity individual gene investigations, and was created to monitor the splicing of 600 cancer-associated genes in 25 normal and 21 serous ovarian cancer tissues. Out of >4,700 alternative splicing events screened, the LISA identified 48 events that were significantly associated with serous ovarian tumor tissues. In a further screen directed at 39 ovarian tissues containing cancer pathologies of various origins, our ovarian cancer splicing signature successfully distinguished all normal tissues from cancer. High-volume identification of cancer-associated splice forms by the LISA paves the way for the use of alternative splicing profiling to diagnose subtypes of cancer.
语言:: eng
DOI:: 10.1158/0008-5472.CAN-07-2580

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