实验库 数据相关信息

题目:
Xylose utilization timecourse
ID:
状态:
发布时间April 9, 2010 , 更新时间 May 2, 2014
物种:
Saccharomyces cerevisiae
摘要:
We have identified a novel xylose-utilization phenotype controlled by a single gene, and we sought to characterize how both the presence and absence of xylose in the presence or absence of the trait affected the S. cerevisiae transcriptional program over time. To do so, we measured global steady state mRNA levels on three pairs of sister spores from a Simi White strain that was backcrossed twice to S288c. Each pair of spores was from an independent tetrad, and contained one xylose utilizing ("positive", GSY2465, 2466, 2469) and one xylose non-utilizing spore ("negative", GSY2464, 2467, 2470). We grew each of the six spores in the presence or absence of xylose for 72 hours with samples taken every 8 hours. We then assayed relative RNA abundance versus a pooled reference, containing equimolar amounts of each sample, using Agilent yeast catalog arrays. time_series_design
实验种类:
transcription profiling by array
样本量:
270
实验设计:
无设计数据
数据号:
E-GEOD-19116, GSE19116
数据状态:

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