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题目:: Shg1p associated RNAs
ID:
状态:: 发布时间June 24, 2010 , 更新时间 May 1, 2014 , 提交时间 March 16, 2009,
物种:: Saccharomyces cerevisiae
摘要:: RNA co-immunopurification with TAP-tagged Shg1p (a component of the Set1 histone methyltransferase complex, SET1C) from Saccharomyces cerevisiae. An untagged (BY4741) served as a control (Gerber et al. 2004). Cells were grown to mid-log phase in rich media and harvested by centrifugation. TAP-tagged Shg1 was affinity-purified from cell-free extracts with IgG sepharose and eluted with TEV protease. RNA was isolated from extract (=input) and from the purified protein sample with the RNAeasy Kit (Qiagen). RNAs were reverse transcribed using a mixture of oligo-dT and random nonamer oligos in the presence of amino-allyl dUTP/dNTP mixture. cDNAs were fluorescently labeled and hybridized on yeast oligo microarrays over night at 42 degrees in formamide-based hybridization buffer. For the method see experimental procedure for RNA IP (Gerber/Dichtl). An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
实验种类:: other
样本量:: 16
实验设计:
: 无设计数据
数据号:: E-GEOD-15247, GSE15247
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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